Lovingly referred to the City of Pearls, this busy southern pride presents numerous charms. Hyderabad is the capital city of southern India’s Telangana state. An important central point for the technology sector, it is a haven for numerous fashionable dining places as well as retail outlets. The historical sites are Golconda Fort, an ancient diamond-trading centre which was after the Qutb Shahi dynastic town. The Charminar, a 16th-century mosque whose four arches aid most elevated minarets, is a traditional city attraction close to the revered Laad Bazaar. Among these picturesque locales live a much-married couple Mr. Ramesh Molugu (name changed) and her loving wife Vanishree Molugu (name changed). Married just 5 years back initially for the first few years to concentrate on their respective careers they didn’t want to start a family. But, now as both are settled in their career with a reputed Indian software company. They started trying to conceive for the past one year but without any success.
Coming from a traditional Hindu family where infertility is considered a taboo, hence it never crossed their minds. They kept on trying for another 6 months but results remained the same. So they consulted their family doctor, who advised them to get a second opinion from the upcoming Indira IVF centre in the city.
Soon enough they were given an appointment at the Indira IVF Hyderabad. The consulting infertility specialist in Hyderabad asked both of them to go through all the necessary tests. Although Vani’s tests came out positive, but Ramesh was detected with a severe male infertility problem.
They were advised to go for an ICSI-IVF fertility treatment and were asked to attend a counselling session at the Indira IVF clinic in Hyderabad with all their queries.
Following are the excerpts of the session wherein queries asked by them and many other couples and answered by our own IVF specialist in Hyderabad Dr. Swati Mothe are collated together in one chapter for the benefit of one and all.
Question: Madam, precisely what is ICSI and how does it help in male infertility? How did it all start? Who was behind this unique technique?
Answer: Intracytoplasmic sperm injection or ICSI is an in vitro fertilization or IVF treatment wherein just one sperm is infused straight into an egg. Faulty sperm functionality continues to be the singular most critical source of male infertility. Even though some serious kinds of male infertility have a hereditary source, the rest might be the consequence of lifestyle issues. In the last decade, ICSI continues to be used more and more around the globe to minimize problems of critical male infertility in patients who often cannot be helped by traditional IVF treatments or perhaps cannot be allowed for IVF since far too few motile as well as morphologically regular sperm were contained in the ejaculate of the male partner, answered Dr. Swati Mothe (Centre Head – Indira IVF centre in Hyderabad).
The very first baby given birth to from a gamete micromanipulation (method wherein exceptional tools, as well as inverted microscopes, are utilized that assist embryologist to select as well as get a single sperm for ICSI IVF) was a baby in Singapore-born in April 1989.
The procedure was developed by Gianpiero Palermo at the Vrije Universiteit Brussel, in the Centre for Reproductive Medicine directed by Paul Devroey together with Andre Van Steirteghem, added Dr. Swati Mothe (Centre Head – Indira IVF clinic in Hyderabad).
Question: How is the ICSI procedure carried out?
Answer: Since Indira IVF, is one of the best IVF centre in Hyderabad, we follow these 5 basic steps to ICSI that include the following: The fully developed egg is supported with a specialized pipette. An exceptionally gentle, pointed, as well as a hollow needle, is employed to immobilize then get just one sperm. The needle is then cautiously put through the shell of the egg and into the cytoplasm of the egg explained Dr. Swati Mothe (Centre Head – Indira infertility clinic and test tube baby centre in Hyderabad).
ICSI is usually carried out following a transvaginal oocyte retrieval process to get one to a number of oocytes from a female.
In ICSI-IVF, the male spouse or perhaps a donor has to give a sperm sample on the very same day while your eggs are gathered. The sample shall be examined in the lab, in case there is absolutely no sperm in his semen, physicians will get sperm from the epididymis or simply testicle. The extraction of sperm from epididymis is usually referred to as (PESA) Percutaneous epididymal sperm aspiration as well as extraction of sperm from testicle is likewise referred to as (TESA) Testicular sperm aspiration and (TESE) Testicular sperm extraction.
The process is conducted under a microscope employing numerous micromanipulation gadgets (micromanipulator, microinjectors and then micropipettes). A controlling pipette stabilizes the fully developed oocyte with mild suction put on by a microinjector. From the opposing side a slim, hollow glass micropipette is employed to collect just one sperm, getting immobilised it by trimming its tail with the tip of the micropipette. The oocyte is pierced by the oolemma then guided to the interior section of the oocyte (cytoplasm). The sperm is then simply discharged into the oocyte. The pictured oocyte bears an extruded polar body system at around 12 o’clock showing its maturity. The polar shape is set at the 12 or 6 o’clock situation, to make sure that the embedded micropipette is unable to disturb the spindle inside the egg. After the process, the oocyte is going to be put into cell culture then examined on the following day for indications of fertilization informed Dr. Swati Mothe (Centre Head – Indira IVF centre in Hyderabad).
Question: What is the IMSI?
Answer: Intracytoplasmic morphologically-selected sperm injection (IMSI) is an improvement of the ICSI method in which sperm samples are inspected under a microscope which is virtually 6000 times more powerful to better evaluate their ‘morphology’.
The Intra Cytoplasmic Sperm Injection also, referred to as IMSI for Intracytoplasmic morphologically-selected sperm injection is a laboratory method utilized in IVF treatments (IVF) and even includes injecting sperm into the egg.
IMSI is a real-time process in which sperm is chosen before the microinjection happens. This is successfully done utilizing an inverted microscope which is in a position to give significantly increased magnifying power (around 6000 times) as compared to that usually found in reproductive laboratories (four hundred times) to perform ICSI.
Using this microscope, Biologists can easily see the inner morphology of sperm as well as reject the ones with irregularities. Having the ability to select sperm without morphological modifications is believed to improve the possibilities of successful implantation of the pre-embryo and also reduce the chance of miscarriage.
This method nonetheless needs more research to establish its effectiveness as well as authenticity so as to be applied routinely in the assisted reproduction laboratory in order to be advised with adequate clinical research, advised Dr. Swati Mothe (Centre Head – Indira IVF centre in Hyderabad).
Question: Madam, what is the difference between ICSI and IVF?
Answer: ICSI is quite the same as traditional IVF in such as gametes (eggs as well as sperm) are accumulated from each spouse. The distinction between the 2 processes is the procedure for attaining fertilisation. In traditional IVF, the eggs, as well as sperm, are combined together in a dish as well as the sperm fertilises the egg ‘naturally’ stated Dr. Swati Mothe (Centre Head – Indira IVFcentre in Hyderabad).
Question: How many sperms are needed for IVF as compared to ICSI?
Answer: The procedures before as well as after insemination are precisely as with a traditional IVF without ICSI, the sole change is the insemination procedure. To carry out ICSI just one sperm is required per egg while doing a traditional IVF without ICSI between 50,000 to 100,000 are required informed Dr. Swati Mothe (Centre Head – Indira IVF centre in Hyderabad).
Question: How much time will it really take for ICSI treatment?
Answer: Being one of the best IVF hospitals in Hyderabad a single process of ICSI takes between 4 weeks or 6 weeks to finish. You and your spouse only need to devote a single day at our facility for the egg as well as sperm retrieval processes. And you will be required somewhere between 2 to 6 days later for the embryo transmission process.
IVF and ICSI are complex clinical procedures, for better clarifications on such issues you can contact our IVF Centre in Hyderabad. You can also follow us on our blog page for more articles related to IVF treatments.
Gynaecologist & IVF Specialist
Gynaecologist & IVF Specialist
Gynaecologist & IVF Specialist